Folder2ISO is a lightweight and powerful application for creating an ISO image, it is a freeware tool which creates an ISO from any kind of folder (including the subfolders).
Click Select Folder to locate the folder which you want to convert. Now click Select Output to select the destination folder where you want to save your ISO file.
If you want, give a label to the ISO file which you are going to create. Now click on Generate ISO, and it will automatically create an ISO from your folder.
To create an ISO image of a CD / DVD drive, you can use the utility LC ISO Creator, simply select the drive you want to create the ISO image file from, then click the Create ISO button and choose the directory where you want to save the file to. That’s it !